cyclin b Search Results


anti cyclin b antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti cyclin b antibody
Anti Cyclin B Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti cyclin b f2f4  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti cyclin b f2f4
Mouse Anti Cyclin B F2f4, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin b polyclonal antibody  (GenScript corporation)
90
GenScript corporation cyclin b polyclonal antibody
Antibodies information in this study
Cyclin B Polyclonal Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin b polyclonal antibody - by Bioz Stars, 2026-07
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mouse anti-cyclin b1 antibody  (Becton Dickinson)
90
Becton Dickinson mouse anti-cyclin b1 antibody
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Mouse Anti Cyclin B1 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclin+b/pmc06041754-238-32-37?v=Becton+Dickinson
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mouse anti-cyclin b1 antibody - by Bioz Stars, 2026-07
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cdk-1/cyclin b, active complex  (Upstate Group Inc)
90
Upstate Group Inc cdk-1/cyclin b, active complex
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Cdk 1/Cyclin B, Active Complex, supplied by Upstate Group Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gns1 conjugated alexa fluor 647 (a647  (Becton Dickinson)
90
Becton Dickinson gns1 conjugated alexa fluor 647 (a647
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Gns1 Conjugated Alexa Fluor 647 (A647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclin+b/pmc03851588-87-17-28?v=Becton+Dickinson
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gns1 conjugated alexa fluor 647 (a647 - by Bioz Stars, 2026-07
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truncated form of xenopus cyclin b  (Pfleger GmbH)
90
Pfleger GmbH truncated form of xenopus cyclin b
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Truncated Form Of Xenopus Cyclin B, supplied by Pfleger GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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truncated form of xenopus cyclin b - by Bioz Stars, 2026-07
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cyclin b  (USA Cycling Inc)
90
USA Cycling Inc cyclin b
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Cyclin B, supplied by USA Cycling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin b - by Bioz Stars, 2026-07
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cyclin b  (Becton Dickinson)
90
Becton Dickinson cyclin b
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Cyclin B, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclin+b/pm22505597-24-27-37?v=Becton+Dickinson
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anti-cyclin b  (GenScript corporation)
90
GenScript corporation anti-cyclin b
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Anti Cyclin B, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin b mm05373 antibody  (Merck KGaA)
90
Merck KGaA cyclin b mm05373 antibody
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Cyclin B Mm05373 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin b mm05373 antibody - by Bioz Stars, 2026-07
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cdk1/cyclin b  (Biomol GmbH)
90
Biomol GmbH cdk1/cyclin b
Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of <t>cyclin</t> <t>B1</t> accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).
Cdk1/Cyclin B, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Antibodies information in this study

Journal: Cell Proliferation

Article Title: Mesentery AjFGF4–AjFGFR2–ERK pathway modulates intestinal regeneration via targeting cell cycle in echinoderms

doi: 10.1111/cpr.13351

Figure Lengend Snippet: Antibodies information in this study

Article Snippet: Cyclin B polyclonal antibody , Rabbit , WB:(1:1000) , GenScript.

Techniques:

Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of cyclin B1 accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).

Journal: Journal of Molecular Cell Biology

Article Title: Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation

doi: 10.1093/jmcb/mjx045

Figure Lengend Snippet: Ran is a novel substrate of TIP60 in mitotic cells. ( A ) Real-time mitosis in Mog1-suppressed cells was scored as a function of TIP60 inhibition by NU9056. Scale bar, 10 μm. ( B and C ) Statistics of the chromosome segregation abnormality in the presence of TIP60 inhibitor (NU9056) or PCAF inhibitor (C146) ( B ) and in cells transfected with TIP60 siRNA ( C ) (mean ± SEM, n = 62, siControl; n = 36, siMog1; n = 55, DMSO; n = 39, C146; n = 38, NU9056; n = 28, siMog1 + DMSO; n = 26, siMog1 + C146; n = 35, siMog1 + NU9056; n = 33, siTIP60; n = 35, siMog1 + siTIP60). Statistical significance was tested by two-sided t -test; ** P < 0.01, *** P < 0.001. ( D ) Representative mass spectra of acK134-Ran from Ran immunoprecipitates isolated from mitotic HeLa cells. ( E ) Characterization of affinity-purified acK134-Ran antibody. Aliquots of HeLa cells were transfected with Ran siRNA followed by nocodazole synchronization or treated with TIP60 inhibitor NU9056. Cells were harvested and lysed in RIPA buffer and the acetylation level of Ran was analyzed by western blot. Note that the reactivity of acK134-Ran antibody was abolished either by treatment with TIP60 inhibitor NU9056 or siRNA-mediated Ran suppression which demonstrates the specificity of acK134-Ran antibody. ( F ) TIP60 or PCAF was incubated with Ran in the presence of Ac-CoA for in vitro acetylation assay. The acetylation level of Ran was analyzed by anti-acK134-Ran antibody. ( G ) Temporal profile of cyclin B1 accumulation, Ran acetylation (acK134-Ran), and TIP60 phosphorylation (pS90-TIP60).

Article Snippet: In western blotting analyses to detect proteins expression level, the following antibodies were used: rabbit anti-Mog1 antibody (sc-292432, Santa Cruz), mouse anti-Ran antibody (sc-58467, Santa Cruz), mouse anti-RCC1 antibody (sc-374325, Santa Cruz), mouse anti-cyclin B1 antibody (554177, BD Pharmingen), mouse anti-α-Tubulin antibody (T9026, clone DM1A, Sigma-Aldrich), mouse anti-active Ran (Ran-GTP) antibody (26915, NewEast Biosciences).

Techniques: Inhibition, Transfection, Isolation, Affinity Purification, Western Blot, Incubation, In Vitro, Acetylation Assay